Part:BBa_K1331004:Design

Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 37
  Illegal NgoMIV site found at 758
  Illegal NgoMIV site found at 871
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 387
  

Design Notes

Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence.

Source

Pseudomonas aeruginosa SQ6